Human adiponectin II. Precautions This reagent is an in vitro test reagent. It is used within the validity period. The reagent should be regarded as an infectious agent. Reagents with different batch numbers cannot be mixed. Before use, the reagents in the box should be taken out and left at room temperature for at least 30 minutes. Store at 2-8 ° C, do not freeze, please see the label in the box for the expiration date. After the concentrated washing solution crystallizes, please incubate at 37 ° C for 15 minutes. 3. Operation steps Add 100 ul of the diluted sample serum and standard respectively to each well. The plate was incubated at 37 ° C for 30 minutes. Shake off the liquid in the plate, wash with the application washing liquid, stay for 30 seconds each time, pat dry on the paper. Repeat the operation 5 times. Add 100ul of enzyme-labeled coupling solution to each well. The plate was incubated at 37 ° C for 30 minutes. Shake off the liquid in the plate, wash with the application washing liquid, stay for 30 seconds each time, pat dry on the paper. Repeat the operation 5 times. Add 50 ul each of Substrate A and Substrate B to each well, and place in a 37 ° C incubator for 15 minutes. 8. Add 50ul of stop solution to each well and read the OD value at 450nm. Human (human) adiponectin 4. Result judgment instrument value: read the OD value of each well on a microplate reader with a wavelength of 450 nm. Detection value range: 0 – 32ug / ml Sensitivity: 0.1ug / ml Melamine melamine antibody 0.2ml Melan-A / MART-1 melanoma-associated antigen / melanin-A antibody 0.2ml MEK1 / MAPKK1 / MEKK1 (mitogen activated protein kinase kinase 1) MEK1 / MAPKK1 antibody (N-terminal) 0.2mlMenin / Men1 Menin tumor suppressor protein antibody 0.2ml Metal ion transporter Arabidopsis metal ion transporter antibody 0.2mlMfn1 (Mitofusin1) Mitochondrial fusion protein 1 antibody 0.2ml IgG (Cell Flow Isotype Control) 0.1mlGoat IgG / APC Fluorescein APC Labeled Goat IgG (Cell Flow Isotype Control) 0.1mlRat IgG / APC Fluorescein APC Labeled Rat IgG (Cell Flow Isotype Control) 0.1ml

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