ELISA test equipmentIn the 1980s, there were many domestic products of ELISA test equipment, mainly divided into two categories: one is continuous micro-sampling, produced by Shanghai No. 3 Analytical Instrument Factory, Jilin Siping Radio Factory, Zhejiang Wenzhou Mould Factory; The other type is to place the entire micro-reaction plate into the tester for measurement, which is produced by the Sichuan Ninth Instrument Factory in Chongqing. Most domestic ELISA testers can meet the prescribed standards. There are also added to the 72 spectrophotometer MB-1 type enzyme-linked plug-in product (492nm). The ELISA tester is based on the color reaction between the enzyme and the related substrate to form an optical density change. It is designed and manufactured using the principle of photoelectric colorimetry. Some people in China call the ELISA tester "" "Enzyme label colorimeter" or "enzyme label quantitative tester", in fact, this is not accurate, because academically "enzyme label" refers to a certain way, the enzyme and antibody (or antigen) protein binding process. 1. How to use the ELISA tester: The ELISA tester should be installed in a dry, clean, and quiet laboratory with relatively stable temperature and humidity. The power supply voltage can be used with alternating current. Use the time-division calibration instrument, test samples, and wash cuvette Link. Correction Instrument: 1. Close the light door, insert the required filter, and turn the range selection switch to T "100%". 2. Turn on the power, the indicator light is visible. 3. Preheat for more than 15min. 4. The instrument is adjusted from the "0" switch to the meter pointer is 0.5, the control solution is injected, the light door is opened, and the dimming amount knob makes the meter pointer 100% full. 6. Turn the range to the desired A (optical density ) Range range, use the A knob to adjust to 0. Determination of the sample: 1, install the pump button, remove the control solution. 2, put down the button switch, use a micro pipette (also called micro liquid sampler) to inject the sample You can read, and the sample test procedure is from thin to thick. Washing the cuvette: turn on the pump and clean the cuvette more than 3 times to test the sample of different components. Second, the auxiliary equipment related to the ELISA tester: In the ELISA measurement, other relevant auxiliary uses are polystyrene-vinyl plastic micro-reaction plates and micro-liquid samplers. In the 1980s, polystyrene plastic micro-reaction plates were produced by Shanghai No. 3 Plastic Products Factory, with different volumes of trace liquid The sampler is produced by Shanghai Qiujing Glass Instrument Factory and other manufacturers. The quality of polystyrene plastic micro-reaction plate and ELISA test result A lot, especially when using the ninth Sichuan Instrument Factory production of ELISA test instrument is more prominent. 21st century with the rapid development of science and technology, ELISA test instrument performance and appearance have undergone great changes.

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