1. Fix the mouse, and straighten and tighten the tail, this is the first step to success.

Mice are more docile, generally do not bite, and are easier to grasp and fix. Usually use the right hand to lift the tail of the mouse and place it on the squirrel lid or other rough surface. When the mouse struggles forward, pinch the skin of both ears and neck with the left thumb and index finger and place the mouse in the left hand. The palm, ring finger and little finger clip the skin and tail of the back to completely fix the mouse. In some extraordinary experiments, such as tail vein injection, you can use an extraordinary fixture to fix it, such as a tail vein injection rack or a thick glass test tube. If you want to perform surgery or heart blood collection, you should first perform anesthesia and then operate. If you perform anatomical experiments, you must first perform painless execution.

Or use this method:

(1) The mouse should be fixed. Make a cage and ventilate the front. It is better to have a baffle in the middle to prevent the mouse from retreating. Open a hole in the back of the tube. The tail comes out from here, so it is fixed firmly

(2) Soak the tail with a little hot water for a few minutes before injection, which is good for injection;

(3) Go far and end, don't start from the root of the tail at the beginning, it is not easy to do that failure;

(4) After entering the blood vessel, pay attention to maintaining stability, and the needle tip easily penetrates the blood vessel. As for whether it can be penetrated, it is up to you to feel how you feel about it. Practicing more, it must be grasped quickly, not difficult, absolutely not difficult! I am more skilled in practicing more than 10 mice

2. Wipe the tail with an alcohol cotton ball to dilate the blood vessels; or use hot water or a hot towel to simmer the heat to dilate the veins; use an appropriate needle, the thinner the better; the injection is closer to the upper part of the tail, where the blood vessels are larger . Wipe with alcohol or hot water. When wiping, you can force the tail to the desktop. The injection state was whitish tail, and two red veins were clearly visible on both sides of the white tailbone.

3. Fix the tail of the mouse with the index finger, middle finger, ring finger and thumb of the left hand; technique: hold 0.1ml in front of the 1ml syringe. Put the little finger of the right hand on the left thumb that pulls the tail of the mouse to insert the needle according to this hand shape; look at the slope in front of the needle tip has 3/4 (critical), if the blood vessel is full, then enter 1/2, enter, stop, pick up the needle, enter the needle ; Swing left and right, if movable, can be injected. The principle is: completely fix the shape of the hand you hit into for the first time, and repeat every time. 4. Injection: The tail is pulled with the left hand during injection, so that the tail is close to the desktop, and the tail and the edge of the table are close to the turning point as the needle insertion site. Generally, the needle is selected 1/4 or 1/3 from the tip of the tail, where the skin is thin The blood vessels are clear and the needle is easy to insert. When the needle is inserted, the needle is parallel to the desktop, the tip of the needle is slightly downward, and the needle is inserted slightly above the point where the middle finger and ring finger touch the thumb. Once entered, the needle must be slightly lifted into the needle, and the needle enters along the blood vessel. If the needle advances in the blood vessel, it can be clearly felt that the needle is flowing smoothly and there is no resistance. If the needle is not in the blood vessel, there is resistance in the feel of the needle, and there is a feeling of blockage. Do not go too deep when inserting the needle. After the needle enters the skin, move the needle slightly upwards and parallelly enter the needle. When the needle penetrates, there is a sense of emptying. When there is no resistance when pushing the liquid, it means success! , Launched again. It is best to enter the needle at 1 / 3-1 / 2 of the tail, and it can be even lower. If you fail this time, you can come back in the past. Do n’t fight at the top right away because you have to fight for so many days. Prepare for the future. With hot water, alcohol, infrared can dilate blood vessels. Can be used as appropriate. In short, the most important thing is "practice makes perfect." You'd better practice first or ask experts to help. Otherwise, in the last few days, it is really difficult to fight.

2. Injection: After entering the needle, remember not to tremble your hands, because the blood vessel wall is very thin and easily pierced. When pushing medicine, push slowly. If the needle is successfully inserted, the medicine is pushed smoothly without resistance; if there is resistance when the medicine is pushed, it means that the needle is not in the blood vessel and it must be pulled out in time and the needle re-entered. Generally choose No. 4 or No. 4 half needles. It is best to use a 2 ml disposable syringe for vaccination. The tail vein injection is not difficult, it is mainly a feeling. If you use ten rats to practice your hands, you will definitely find the feeling. Keep your mind calm, your hands steady, and be patient.

5. Test whether the needle is in the vein by looking for blood return;

6. Injection. Do the above steps to ensure successful injection!

HAMILTON micro syringe

Note:

(1) If you do cell transplantation, try to break up the cells as much as possible, otherwise it will easily cause embolism!

(2) Practice: It is recommended that you practice your hands with blue ink first, because novices often ca n’t see whether they are under the skin or veins. In fact, they can only really make good practice. It is generally recommended to insert the needle at the lower third of the tail, but I think 1/2 The accuracy is good. Personal experience can insert the needle deeper to prevent accidental swinging to pierce the vein and inject it under the skin. Many times you think that the blood is injected into the blood vessel but the skin is for this reason.

Or use: It is often easier to inject placenta orchid through the tail meridian. The one with the veins on the side is thicker. You can make a container. We use a 50ml centrifuge tube to make an opening in the front and a screw cap in the back to make a hole to hold the mouse. It's relatively easy to get in. It's easy to put the mice into the tube and put the tail through the hole in the lid. Shave, scald it with hot water, and the blood vessels swell up. The specific feeling should be practiced and grasped by yourself, but it is easy, and there are 3-4 can be grasped.

(3) Rule: The smaller the animal, the better the operation. The mouse is easier than the rat, and the young rat is easier than the adult rat; it is best to use the tail vein injection for the mouse, so that the damage is minimal; the adult rat ca n’t choose the stock. Intravenous injection is relatively easy; the most important thing is to practice more. The feeling when entering the needle must be understood by yourself, and practice makes perfect. This is common sense.

Skillful skills are important, and precision syringes are also essential to obtain good data. HAMILTON micro-syringe is the world's top brand. She uses high-quality materials and skilled manufacturing processes, so that each product produced has the unique characteristics of high performance and high precision, and meets the requirements of customers in the field of fluid precision measurement. The accuracy of the Hamilton syringe is within ± 1% of the nominal value, and the product quality is certified by EN ISO 9001 / EN 46001.

Shanghai Hengyuan Biological Technology Co., Ltd.'s main products: ELISA kits, biological reagents, serum, antibodies, standards, etc., kits for free testing, as long as you send us the specimens, we guarantee the results, please contact us for more information advisory.

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