Human transfer chemoattractant growth factor B1 2. Notes 1. This reagent is an in vitro test reagent and is used within the validity period. The reagent should be regarded as an infectious agent. Reagents with different batch numbers cannot be mixed. The reagents in the box should be taken out before use. Leave at room temperature for at least 30 minutes. After the concentrated washing liquid crystallizes, incubate at 37 ° C for 15 minutes. After the concentrated sample diluent crystallizes, please incubate at 37 ℃ for 15 minutes. If the experiment is performed within 24 hours, the specimen can be stored at 2 ～ 8 ℃. No need for timely experiment, the specimen should be stored at -20 ℃ to avoid repeated freezing and thawing. After repeatedly cleaning the microwell plate, and buckle the residual liquid in the microwell, otherwise it will reduce the accuracy and cause the false image of absorbance deviation. After the sample is added, the microwell reaction strip should be shaken slightly to mix the liquid in the well. The kit is stored at 2 ～ 8 ℃, please do not freeze it, please refer to the label in the box for the expiration date. Human transfer chemotactic growth factor B1 III. Preparation before the experiment 1. Before use, the reagents in the box should be taken out and left at room temperature for at least 30 minutes. 2. 2. Prepare various experimental instruments and materials, such as micropipettes, pipette tips, medical distilled water, etc. 3. 3. Concentrated lotion and medical distilled water are diluted 1:19 times to become the applied lotion 4. 4. Dilute the concentrated sample diluent and medical distilled water 1: 4 times into the applied sample diluent 5. Dilute the sample with the application sample diluent, dilute the sample according to the volume ratio of 1: 100, for example, 10μl of the sample is added to the 1ml application sample diluent, and mix well to use. 4) Operation steps Remove the enzyme labeling plate, set up blank wells, add 100μl of standard products to the blank microwells according to the order (the blank wells are regarded as No. 0 standard, and use medical distilled water to replace) 2. Mark the sample number separately and add 100μl of diluted sample In blank microwells (different samples use different tips). 3. Incubate the microtiter plate at 37 ° C for 30 minutes; 4. Remove the microtiter plate and shake off the liquid. After each well is filled with application washing liquid, immediately shake off the liquid; 5. After each well is filled with the application washing solution, shake the enzyme plate slightly for 30 seconds, then shake off the application washing solution in the wells, and pat the enzyme plate on the absorbent paper to dry. 6. Repeat the fifth step 5 times, pat the enzyme plate dry on absorbent paper. 7. Add 100 μl of enzyme-labeled coupling solution to the standard well and sample well. 8. Incubate the 96-well plate at 37 ° C for 30 minutes. 9. Take out the enzyme labeling plate and shake off the liquid. After all the application washing liquid is filled in each well, immediately shake off the liquid. 10. After refilling each well with the washing application solution, shake the enzyme plate slightly at room temperature for 30 seconds, then shake off the application washing solution in the wells, and pat the enzyme plate on the absorbent paper to dry. 11. Repeat the fifth step 5 times, pat the enzyme plate on the absorbent paper to dry. 12. Add 50 μl of substrate A to each well immediately after adding 50 μl of substrate B. Gently shake to mix. (Liquid A and B are added with different tips) 13. Incubate the microtiter plate at 37 ° C in the dark for 15 minutes. 14. Add 50 μl of stop solution to each microwell and gently shake to mix. 15. Measure OD at 450nm on a microplate reader; after color development, measure within 15 minutes. 16. According to the prepared standard curve, calculate the sample content of human chemotactic growth factor B1. V. Judgment of results 1. Instrument value: read the OD value of each well on a microplate reader with a wavelength of 450 nm; 2. Range of detection value: 0 －160pg / ml 3. Sensitivity: 1.0pg / ml Nociceptin receptor 0.2ml NogoR / NGR axonal hyperinhibitor receptor / Nogo receptor antibody 0.2ml Bovine IgM / PE fluorescein PE-labeled bovine IgM 0.1ml Chicken IgG / PE Fluorescein PE-labeled chicken IgG 0.1ml Chicken IgM / PE Fluorescein PE-labeled chicken IgM 0.1mldog IgG / PE Fluorescein PE-labeled dog IgG 0.1ml Nogo-A / NEP Axonal hypergrowth inhibitory factor- A antibody 0.2ml human GAPDH polyclonal antibody / FITC fluorescein FITC labeled anti-human GAPDH polyclonal antibody 100T

Wine Box

Wine box, usually refers to the box that holds bottled wine. Materials are: Wooden Box, leather box, metal box and paper box. Different shapes for shock and decoration.

Wine box, also known as red wine box, is usually called wine packaging box or red wine packaging box. As the name implies, it is the outer packaging of wine (red wine), which has the function of protecting red wine for convenient transportation and storage, and at the same time, it is beneficial to wine ( red wine).

1. Wine wooden box (red wine wooden box)
Usually include log wine boxes (multi-purpose materials include pine, paulownia, etc.), imitation mahogany wine boxes (multi-purpose materials include MDF, etc.), and the shapes are diverse and are mostly used for medium and high-end wine packaging.

2. Wine leather box (red wine leather box)
Most of the wine leather boxes (red wine leather boxes) are made of PU, PVC and other artificial leather. The leather packaging is more suitable for the characteristics of wine, and can be divided into single-pack, double-pack, four-pack and six-pack in terms of capacity.

3. Metal wine box (metal wine box)
There are few metal wine boxes in the market and are not widely used. Most of them are made of aluminum alloy, iron, and other alloys.

4. Wine carton (red wine carton)
Wine cartons are mostly made of cardboard, corrugated paper, special paper, etc. Since the raw materials are paper, which is convenient for secondary recycling, the shape can be diversified, and other processes are convenient, so wine cartons are widely used in wine packaging.

Wood Wine Box,Wine Sotrage Box,Custom Logo Wine Box,Wine Bottle Box,Wine Gift Packaging Box,Wine Bottle Box

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