The solid phase extraction device produced by Shanghai Haozhuang is exquisite in appearance and simple in operation
Shanghai Haozhuang Instrument Co., Ltd. has always followed the guidelines of survival by quality, development by science and technology, efficiency by management, and reputation by service. , Is willing to win the trust of the majority of users with the best quality and service. The instrument products independently developed by Shanghai Hao Zhuang (LNB) Company are well received by users for their beautiful appearance, compactness, practicality and low price. It can also be equipped with Haozhuang PS series vacuum pumps to achieve the purpose of pressure extraction. Shanghai Haozhuang teaches you how to operate the solid phase extraction device correctly. The operation procedure of solid phase extraction is divided into the following steps:
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â‘ Weak polar or non-polar adsorbents used in reversed-phase solid phase extraction are usually rinsed with water-soluble organic solvents, such as methanol, and then rinsed with water or buffer solution. It can also be rinsed with a strong solvent (such as hexane) before being rinsed with methanol to eliminate impurities adsorbed on the adsorbent and its interference with the target compound.
â‘¡ The polar adsorbent used in normal phase solid phase extraction is usually eluted with the organic solvent (sample matrix) where the target compound is located.
â‘¢ The adsorbent used for ion exchange solid phase extraction can be rinsed with the sample solvent when used in samples in non-polar organic solvents; after being rinsed with water-soluble organic solvents when used in samples in polar solvents Then, rinse with an aqueous solution of appropriate pH and containing certain organic solvents and salts.
In order to keep the adsorbent in the solid phase extraction cartridge wet after activation until the sample is added, approximately 1 ml of the activation solvent should be kept on the adsorbent after activation.
2. Load the sample. Pour the liquid or dissolved solid sample into the activated solid phase extraction cartridge, and then use the vacuum and pressure to make the sample enter the adsorbent.
3. Washing and elution After the sample enters the adsorbent and the target compound is adsorbed, the weak retention interfering compound can be washed away with a weaker solvent, and then the target compound can be eluted with a stronger solvent and collected. The elution and elution are the same as described above, and the eluent or eluent can be passed through the adsorbent by vacuuming, adding or centrifuging.
If, when choosing an adsorbent, you choose to adsorb the target compound very weakly or not, and when the adsorbent has a strong adsorption for the interfering compound, you can also let the target compound elute and collect it, so that the interfering compound remains (adsorption ) On the adsorbent, the two are separated. In most cases, the target compound is retained on the adsorbent, and finally eluted with a strong solvent, which is more conducive to sample purification.
In order to facilitate the use of solid phase extraction, Shanghai Haozhuang Instrument has also developed a lot of special equipment for solid phase extraction, making solid phase extraction more convenient and simple to use. A solid phase extraction device (SPE) is a physical extraction process that includes a liquid phase and a solid phase. In the extraction process, the adsorption force of the solid to the analyte is greater than that of the sample mother solution. When the sample passes through the SPE column, the analyte is adsorbed on the solid surface, and other components pass through the column with the sample mother solution. Eluted. SPE is widely used, such as analysis of biological fluids including blood, urine, serum and plasma and cytoplasm; analysis of milk processing, alcohol, beverages and juice; analysis and monitoring of water resources; fruit and vegetable grains and various plant tissues and Animal tissue; tablets and other solid drugs. Analysis of pesticide and herbicide residues in fruits, vegetables and food, analysis of antibiotics and clinical drugs, etc.
The laboratory instruments and equipment produced by Shanghai Haozhuang win the market by quality and customers by credibility. For more high-quality products, please pay attention to Shanghai Haozhuang!
