The necessary step in the tumor research is to homogenize the pathological tissue before using it in the follow-up research. One method is to use ophthalmic scissors to shred the tissue first, and then homogenize with a knife head or tank homogenizer.

The blade-type or pot-type homogenizer uses shearing, mashing or grinding to homogenize the animal and plant tissues to obtain the cytoplasm and mitochondria required for the experiment. There are two major problems when using this kind of homogenizer: first, it needs to be cleaned after homogenization, and if necessary, high temperature and high pressure sterilization; second, the loss of samples, if the amount of tissue sampled is small, such as obtained by puncture A small amount of tumor tissue, using this kind of homogenizer will inevitably lose part of the sample.

The flap-type tissue homogenizer avoids the above two problems very well. In the test, a sterile plastic bag is used to hold the samples. To homogenize different samples, only the sterile plastic bag needs to be replaced. There is no need to clean the instrument and the instrument. Sterilization treatment improves test efficiency, avoids sample loss, guarantees sample quality and safety, and also ensures the safety of the laboratory environment. Beating homogenizer-this kind of homogenizer box has two pedal-shaped paddles reciprocating, put the sample in a sterilized plastic bag, and then put it into the homogenizer, according to the tissue sample The degree and size of softness and rigidity are used to adjust the strength and speed of homogenization. The paddles alternately flap the plastic bag to break up and mix the sample.

At present, there are many major brands of beating homogenizers on the market, such as Seward, Interscience, IUL, and domestic brands such as Xinzhi, etc. Most of them are classified by sample processing volume. Among them, the Stomacher microBiomaster developed by Seward has a sample processing capacity of up to 250 μl and up to 80ml. It is a compact, flexible and efficient tissue homogenizer that can be placed in a biological safety cabinet for operation. It is widely used in clinical, In the fields of medicine, life sciences and other fields such as living tissue processing, sputum processing, tissue culture and cell biology, etc. in experimental operations. In addition, the tapping homogenizer has also developed models with heating or cooling to meet the requirements of different experiments. For example, the Stomacher 3500 Thermo Biomaster from Seward and the JumboMix3500 WarmMix from Interscience both support the heating function, and the heating temperature ranges are 20 ~ 40 ° C and 20 ~ 50 ° C respectively. However, water cooling is used to achieve cooling, and the cooling temperature is limited by the temperature of the circulating water. Therefore, some organizations can choose this type of refrigeration if they need low temperature conditions in the sample preparation process, but the current flapping homogenizer still needs to be improved in terms of refrigeration technology. With the continuous improvement and improvement of the design technology of the beating homogenizer and the continuous strengthening of the understanding of laboratory biosafety, the beating homogenizer is bound to be more and more favored by the majority of laboratory staff, and will be more widely used in various Classes that require homogeneous homogenization, such as tumor tissue sample preparation experiments.

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