Shanghai Haozhuang Instrument teaches you how to better use the biological safety cabinet
Shanghai Haozhuang Instrument Co., Ltd. has developed low-temperature and constant-temperature instruments, ultrasonic instruments, cabinet instruments, microbiological instruments, ice-making refrigeration instruments, laboratory supporting instruments, and gradually formed a variety of scientific instruments and technical reserve products. Product layout. Haozhuang brand biological safety cabinet developed by our company 1. Before operation, you must thoroughly understand the relevant knowledge of the biosafety laboratory, the operating procedures of the biosafety cabinet and the operating methods of the equipment required in the operation. 2. After confirming that the glass window is in the closed position, turn on the UV lamp to sterilize the working space in the safety cabinet. After sterilization, turn off the UV lamp. Lift the glass door to the normal working position. Turn on the fluorescent lamp and fan. 3. Check the return grille so that it is not blocked by objects, and pay attention to the service life of the filter membrane. Without any obstruction, let the safety cabinet work for at least 15 minutes. 4. Wash hands and arms thoroughly with disinfectant. Wear a long-sleeved work gown with elastic cuffs, rubber gloves and sleeves on the cuffs, wear protective glasses, and if necessary, wear a protective mask. Wipe the inner surface of the safety cabinet with 70% ethanol or neutral disinfectant, and then wipe the ethanol or neutral disinfectant clean with pure water and let it dry. The experimental materials must be put in accordance with the experimental procedures. Try to put all the required items into the safety cabinet before the formal operation, but do not overload and do not block the front and rear vents. 5. After putting in the experimental materials and equipment, wait 2-3 minutes to remove the air dust in the work area. 6. Keep all the experimental materials at least 4 â€away from the glass door. Put the contaminated items behind the work area as much as possible. Keep the contaminated materials and clean materials in the work area separate. Try not to put the contaminated materials Into a clean area. 7. During the operation, avoid moving materials casually, avoid frequent movement of the operator's arm in the front opening; minimize the interference of airflow, and try to avoid people entering or leaving the room or walking behind the operator when working. Try not to use open flames. Use appropriate sterilization techniques. Avoid using devices and procedures that can interfere with the airflow in the safety cabinet. If there is material spillage or liquid spills, be sure to disinfect the surface before removing the items from the safety cabinet. In order to prevent any residual contaminants in the safety cabinet, the safety cabinet must be All surfaces are disinfected. 8. After all work is completed, let the safety cabinet continue to work for at least 5 minutes without any obstruction to remove floating pollution in the work area. 9. All objects that come into contact with contaminated materials should be surface disinfected before being removed from the safety cabinet. Cover all open containers before removing them from the safety cabinet. Wipe the inner surface of the safety cabinet with 70% ethanol or an appropriate neutral disinfectant, and then wipe the ethanol or neutral disinfectant with pure water and let it dry. 10. Turn off the fluorescent lamp and safety cabinet fan, close the glass door, and turn on the UV lamp for disinfection. After sterilization, turn off the UV lamp. 11. Periodically lift the lower panel of the work area and wipe or rinse the space under the work surface.
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