Rabbit Intercellular Adhesion Molecule 3 (ICAM-3/CD50) ELISA Kit Instructions for Use
April 08 09:01:54, 2021
Elisa kit specifications: 48-well configuration / 96-well configuration standard dilution: 1.5ml × 1 bottle of enzyme standard reagent: 3 ml × 1 bottle (48) / 6 ml × 1 bottle (96) [rabbit intercellular adhesion molecule 3 (ICAM-3/CD50) elisa kit] This reagent is only for research use calculation: the concentration of the standard is the abscissa and the OD is the ordinate. The standard curve is drawn on the coordinate paper. The standard is based on the OD value of the sample. Curve the corresponding concentration; multiply by the dilution factor; or calculate the linear regression equation of the standard curve using the concentration of the standard and the OD value, substitute the OD value of the sample into the equation, calculate the sample concentration, and multiply by the dilution factor. This is the actual concentration of the sample. Kit composition: sealing film: 2 pieces (48) / 2 pieces (96) Instructions: 1 part sealed bag: 1 standard: 2700ng / L 0.5ml × 1 bottle 0.5ml × 1 bottle 2-8 ° C preservation enzyme Standard package: 1×48 1×96 2-8°C Preservation of sample dilution: 3ml×1 bottle 6 ml×1 bottle 2-8°C Preservation of developer A: 3ml×1 bottle 6 ml×1 bottle 2 Store chromogen B solution at -8 °C: 3ml × 1 bottle 6 ml × 1 bottle 2-8 ° C Preservation solution: 3ml × 1 bottle 6ml × 1 bottle 2-8 ° C Preservation concentrated washing solution: (20ml × 20 times) ×1 bottle (20ml×30 times)×1 bottle 2-8°C preservation experiment principle: The kit uses double antibody sandwich method to determine the level of intercellular adhesion molecule 3 (ICAM-3/CD50) in rabbits. The microporous plate was coated with purified rabbit intercellular adhesion molecule 3 (ICAM-3/CD50) antibody to prepare a solid phase antibody, and intercellular adhesion molecule 3 (ICAM-3) was sequentially added to the microcapsule of the coated monoclonal antibody. /CD50), and then combined with HRP-labeled intercellular adhesion molecule 3 (ICAM-3/CD50) antibody to form an antibody-antigen-enzyme-labeled antibody complex, which was thoroughly washed and then added with substrate TMB for color development. TMB is converted to blue under the catalysis of HRP enzyme and converted to the final yellow color by the action of an acid. The color depth is positively correlated with the intercellular adhesion molecule 3 (ICAM-3/CD50) in the sample. The absorbance (OD value) was measured by a microplate reader at a wavelength of 450 nm, and the concentration of rabbit intercellular adhesion molecule 3 (ICAM-3/CD50) in the sample was calculated by a standard curve. Purpose: This kit is used to determine the content of intercellular adhesion molecule 3 (ICAM-3/CD50) in human serum, plasma and related fluid samples. Service commitment: ? Delivery period: payment to delivery. Free technical consultation and guidance during working hours The company provides biological reagents, Elisa kits, quality assurance, and authoritative suppliers of scientific reagents, which are your trusted choice! Welcome to inquire, 668899055380
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