Monkey Tumor Necrosis Factor Alpha Kit Instructions

Experimental principle of monkey tumor necrosis factor alpha kit This kit uses the double antibody sandwich method to determine the level of monkey tumor necrosis factor alpha (TNF-α) in specimens. The purified monkey tumor necrosis factor alpha (TNF-α) antibody was used to coat the microplate to make a solid-phase antibody. Tumor necrosis factor alpha (TNF-α) was sequentially added to the monoclonal antibody-coated microwells and then labeled with HRP The tumor necrosis factor α (TNF-α) antibody binds to form an antibody-antigen-enzyme-labeled antibody complex, and after thorough washing, the substrate TMB is added for color development. TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid.

The color depth is positively correlated with the tumor necrosis factor alpha (TNF-α) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of monkey tumor necrosis factor α (TNF-α) in the sample was calculated by a standard curve. Hengyuan Biology specializes in supplying high-quality ELISA kit Kit. With its advantages of high quality, high quality and low price, it is favored by universities, research institutes, hospitals, disease centers, etc. across the country; Hengyuan has a group of skilled teams for Efforts to build China's first brand ELISA manufacturer; spot agents of elisa kits are in Hengyuan Bio.

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