How to use ELISA kit
How to use ELISA kit 1. Serum: Avoid any cell stimulation during the operation. Use test tubes free of pyrogens and endotoxins. After collecting blood, centrifuge at 1000 × g for 10 minutes to quickly and carefully separate the red blood cells. 2. Plasma: EDTA, citrate and heparin plasma can be used for detection. Centrifuge at 1000 × g for 30 minutes to remove particles. 3. Cell supernatant: centrifuge at 1000 × g for 10 minutes to remove particles and polymer. 4. Tissue homogenate: Add tissue to normal saline and mash. Centrifuge at 1000 × g for 10 minutes, and take the supernatant. 5. Storage: If the sample is not used immediately, it should be divided into small parts and stored at -70 ℃ to avoid repeated freezing. If possible, do not use hemolysis or hyperlipidemia. If there are a large number of particles in the serum, centrifuge or filter before testing. Do not thaw at 37 ° C or higher. Thaw at room temperature and ensure that the sample is thawed evenly and adequately.
Outside Basketball Court
Outside Basketball Court Basketball Court,PVC Vinyl Basketball Court,Outdoor Basketball Court,Basketball Court Flooring Shijiazhuang Enlio Sports Goods Co., Ltd. , https://www.enliosports.com
Soft connection I and II are ideal for the outside basketball courts. Anti-slippery surface design keeds every champions playing without slipping. New design adjustable buckles to control the thermal expansion. Our Court Tiles is official used by the Serbia National Basketball Federation and FIBA33 World Tour China Zone.
ENLIO provides services from design to manufacture, sales and after sales etc. We are professional in sports floorings, for the outdoor interlocking tiles, we offer perfect solutions for your courts. Based on ISO9001 quality system, products quality are controlled on each link. Your safety and sports comfortability are always our goal.
ADVANTAGE
1.Durable:Suspended structure,interlocking floor.
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3.Long Service Life: 8 years
4.Installation: Easy to install, no need adhesive.
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6.Maintenance: Free maintenance.
7.All Weather: No special weather be required.
8.Tempreture: -40℃-80℃.
9.Certificate: CE, SGS.
10.Multi-purpose and colorful appearance.