How to make the shape of the cell well and more beautiful
How to make the shape of the cell well and more beautiful 1. Different cells, the environment you like is different. This is not just about the difference in culture medium, but also the spatial density of cell growth. This is actually a way to improve. For adherent cells, if the cell morphology is not good (or the cell shape is not clear, the surface seems to have foreign bodies, etc.), the following operations can be performed during the passage: 3. Question about the amount of medium added to the culture flask This is to find out the cells you have raised on your own. Not a small glass square bottle 12ml, a generous bottle 14ml. Some cells, on the other hand, have less media and the cell morphology will grow better. (It may be a big competition, there is a survival of the fittest. Ha ha.) For cells with fast growth, it is better to add less media to the cells that are easy to grow. But pay attention to the liquid change mastery. 4. Questions about choosing a culture bottle Individuals have found that cells that grow faster are growing better in glass bottles than disposable plastic bottles. For the same cell, the growth state in the glass bottle is better than that in the plastic bottle during the period of vigorous growth. This may be because plastic bottles are easier to stick to than glass bottles. Cells that grow fast are not as good as glass bottles in a relatively "comfortable" environment in plastic bottles. 5. Digestion problems during passage It can be said that for cells that need to be digested, each digestion is the most crucial test for the survival or not of this cell. Cell culture series related products Item number product name specification feature Packing quantity Unit price¥ FCL price¥ C81-CTF010150 Culture fluid bottle 150ml Polystyrene material, resistant to dilute acid, disinfected 1 / bag, 24 / box 10.94 197.25 C81-CTF010250 Culture fluid bottle 250ml Polystyrene material, resistant to dilute acid, disinfected 1 / bag, 24 / box 12.03 216.75 C81-CTF010500 Culture fluid bottle 500ml Polystyrene material, resistant to dilute acid, disinfected 1 / bag, 24 / box 13.12 236.25 C81-CTF010001 Culture fluid bottle 1000ml Polystyrene material, resistant to dilute acid, disinfected 1 / bag, 24 / box 30.62 551.25 C81-CTF010002 Culture fluid bottle 2000ml Polystyrene material, resistant to dilute acid, disinfected 1 / bag, 12 / box 39.37 354 Baby sling offers a fashionable, functional baby carrying option for all parents, offers both front and hip-carrying options. Adjusts to grow with baby, newborn to toddler. While the Baby Carrier is simple and stylish, it also enhances safety performance and reduces potential safety hazards. Joybabies offer OEM and ODM to suit difference requirements. Baby Sling Newborn,Baby Sling,Baby Carrier Sling,Baby Sling Carrier JOYBABIES PRODUCT CO.,LTD. , https://www.my-joybabies.com
Some cells are a little more growable and have a better growth state. This is generally a cell that grows slowly. Such as endothelial cells. Some of them are better cells, such as macrophages and certain tumor cells. Especially for macrophages, the growth rate is very fast and the adherence speed is very fast, so a small amount of cells should be left in the passage, so that the cell state will be better. And macrophages prefer to grow together, and there is space between the heap and the heap. If you grow into a full piece, the cell morphology will be basically poor, and there will be more aging, which is not good for the later experimental results. Therefore, when culturing cells, you should explore the density of growth space that the cell likes.
2. The problem of how the cell morphology is not good
First, pour off the old medium, add 3ml of new medium (with or without serum), wash it once, use a dropper to suck away, then add 3ml of medium, pre-blow, control the intensity of the blow, gently The ground is probably passed along the bottom of the bottle and then sucked away. At this time, official digestion and blows began. (Macrophages we only blow, not digest)
Next, the blown cell suspension is added to a new culture flask, and the culture flask is previously added to the culture medium, cultured in an incubator, and the cell adherence is observed at a time point. Observe once every 10 minutes, 20 minutes, and observe once every 30 minutes. Select a time point, and if some cells have been attached to the wall, re-dispose it on the clean bench, quickly pour out the medium from the bottom side, add 3ml of new medium and wash gently. Then add complete medium culture. Subsequent observation of cell growth and morphology. I call it "two passes." Ha ha.
If the effect is not ideal once, it can be repeated multiple times. Until you find the perfect shape of the cell. One should pay attention to the combination of cell-like growth. If you like a little more well-grown cells, you will pass it when the adherent cells are more. vice versa. This was invented by my brother, thank you. This method is really good!
Therefore, if the cells with slow growth rate want a more beautiful cell state, the plastic bottle will be better than the glass bottle, and for the slow growth cell, the glass bottle will be better. Similarly, for the same cell, when it grows slowly, the plastic bottle will be better, such as when it is just revived, or when it is cultured. In the case of its strong growth, the glass bottles will be relatively better.
Many students have encountered this problem. The generations of their own cells have been quite good for generations. However, after several generations, they found that their cells are not working, and the state is getting worse. For this problem, you can say with certainty that there is something wrong with your digestion. Every time you digest, the cells are greatly damaged. So, the longer the worse.
In the process of digestion, after you add trypsin, all the cells are digested by trypsin, but we ignore the problem that the growth state of the cells is different, and the adhesion of each cell is different. Some stick to the wall, some stick to the wall is not so strong, so it is unfair to let all the cells digest the same time, they will be treated differently, of course, there will be temper. . . Ha ha. I later improved the method of digestion. Strive to teach students in accordance with their abilities. I call it the "four-step digestion method." (taking indigestible cells as an example)