The inverted microscope consists of the same microscope as the normal microscope, except that the objective lens and the illumination system are reversed. The former is under the stage and the latter is on the stage for viewing the cultured living cells with a phase difference objective.

The inverted microscope and the magnifying glass play the same role, which is to make a close-up of a small object into an enlarged image for human eyes to observe. Only a microscope can have a higher magnification than a magnifying glass.

The object is in front of the objective lens, and the distance from the objective lens is larger than the focal length of the objective lens, but less than twice the focal length of the objective lens. Therefore, after it passes through the objective lens, it will inevitably form an inverted magnified real image AB. AB is close to the position of F2. After the eyepiece is enlarged to the virtual image AB, it is observed by the eye. The eyepiece acts like a magnifying glass. The only difference is that the eye sees through the eyepiece is not the object itself, but the image that has been magnified once by the objective lens.

Inverted microscopes are used by medical and health units, universities, and research institutes for the observation of microorganisms, cells, bacteria, tissue culture, suspensions, sediments, etc., and can continuously observe the process of proliferation and division of cells and bacteria in the culture solution, and Take any form of this process. It is widely used in the fields of cytology, parasitology, oncology, immunology, genetic engineering, industrial microbiology, and botany.

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