Experimental conditions:

Wavelength: 280nm Flow rate: 1.0 ml / min
Mobile phase: A: 0.2% HAC B: acetonitrile

0.01 92 8

11 85 15

13 82 18

15 80 20

22 82 18

23 85 15

30 92 8
Column: Chenhang Column 88082545 C18 5µ 250 × 4.6mm 001203

Instrument used: CoMetro 6000 high performance liquid chromatograph

--- 6000 LDI precision constant flow pump

--- 6000 PVW UV detector

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