1. Basic Principles of Chromatography (Chromatography) Method 1. Basic Principles of Chromatographic Analysis Method The flowing carrier (mobile phase) "pushes" the analyzed sample (mixture or pure substance) forward (movement). During the movement, the sample being analyzed is continuously exchanged (distributed) between the mobile phase and the stationary substance (stationary phase) in contact with the mobile phase. Due to the different ability of the measured components to interact with the mobile phase and the stationary phase, the speed of their forward movement will be different. In the case of a certain movement distance, the time for different components to reach the end point will be different, so as to achieve the effect of separating the mixture.
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