It's another new working day. Monday morning looks exceptionally good. Today we share with friends what the latest ELISA kit is made of. Our company guarantees 100% of the kit quality. We return and exchange unconditionally. Its main groups are: antigens, antibodies, solid-phase carriers, coatings, enzymes and substrates.

1. Classification and requirements of antigens: There are three main categories, natural antigens, artificial antigens and genetically recombinant antigens. High specificity, affinity and purity are required.

2. Antibodies are divided into polyclonal antibodies and monoclonal antibodies.

3. Enzymes and substrates The most commonly used enzymes in ELISA are HRP and ALP. HRP is a complex enzyme that combines a main enzyme and a prosthetic group to form a porphyrin protein. ALP is a phosphate ester hydrolase, and the ALP activity used as a tracer enzyme should be above 1,000 U / mg.

4. The main means for solid phase carrier to separate the binding marker and free marker should have good binding performance with various immunoactive substances and not change its immunological activity.

5. The process of binding the immunologically active substance to the solid phase carrier is called coating. Commonly used methods include adsorption method, chemical cross-linking method and avidin-biotin indirect coating method. Due to the high affinity between avidin and biotin, antigen or antibody is indirectly bound to the avidin-coated solid Phase on.

If you still do n’t understand or need more information, you can call the manager of our company, and you can leave a message on this website. We will reply to you as soon as possible within half of the work. To provide you with satisfactory ELISA kits is the wish of all employees of Shanghai Hengyuan.

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