PB and PBS are the most commonly used buffers for immunocytochemistry experiments.

0.01mol/L PBS is mainly used for rinsing tissue specimens, diluted serum, etc., and its pH should be between 7.25 and 7.35, otherwise it needs to be adjusted.

0.1 mol/L of PB is commonly used to prepare fixatives, sucrose, and the like.

Under normal circumstances, the pH of 0.2mol/l PB is slightly higher. When diluted to 0.01mol/L PBS, the required pH can often be reached. If pH is to be adjusted, the pH of PB is usually adjusted.

Pbs buffer preservation method

Add about 800 mL of deionized water to dissolve thoroughly, then add concentrated hydrochloric acid to adjust the pH to 7.4, and finally make up to 1 L. After autoclaving, place it in a refrigerator at 4 ° C for use.

PBS buffer (pH 7.2 ~ 7.4): NaCl137mmol / L, KCl2.7mmol / L, Na2HPO4 10mmol / L, KH2PO4 2mmol / L

PBS buffer is generally used as a solvent to dissolve the protective reagent. Specific reagents generally have different proportions of formulations, and have better effects in terms of specificity.

The 1x PBS buffer is 0.01M phosphate buffer and can be used directly. 2x PBS is twice the concentration and is diluted twice when used. 0.1 M PBS is generally not used to configure buffers for other uses.

Phosphate Buffer (PB)

Reagents: NaH2PO4?2H2O Na2HPO4?12H2O

Preparation method: When preparing, first prepare 0.2mol/L NaH2PO4 and 0.2mol/L Na2HPO4, and mix them in a certain ratio to form 0.2mol/L phosphate buffer (PB), and prepare different concentrations as needed. PB.

0.2mol/L phosphate buffer (pH 5.7 ~ 8.0)

The PBS is phosphate buffered saline (phosphate buffered saline) 0.01mol / L phosphate buffered saline: Nacl 8 g, Na2HPO4.12H2o 3.48g, KH2PO40.02g, Kcl 0.02g, placed in a 1000ml volumetric flask, fully Dissolve, finally set to 1000ml.

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